Microblot–Array (MBA) is a new generation of unique, immunoblot array in a microtiter plate format. It is designed for efficient multiplex diagnostics, enabling the simultaneous detection of multiple markers which can save time and lower costs. The technology eliminates the limited capacity of traditional BLOT strips and opens the opportunity for high throughput testing.
The main benefit of Microblot-Array kits is the high number of antigens which can be simultaneously detected in one sample. The kits are primarily intended for confirmation of ELISA or other screening methods. However, they also enable identification of specific antibodies and thus differentiation of systemic autoimmune diseases, such as myositis, scleroderma, systemic lupus, and others. The kits are optimized and validated for detection of specific IgG in human serum or plasma.
Main clinical areas covered:
- Infectious serology
- Autoimmunity
Microblot-Array
- Antigens spotted in triplicate – minimizing statistical variation
- Controls in each well
- 4 calibration spots to create a calibration curve
- Evaluation based on combination of positive antigen spots: qualitative, quantitative (U/ml) or semiquantitative (IP)
Microblot-Array Software
- Automated test identification
- Intuitive and user-friendly guiding throughout the results evaluation
- Complex image analysis
- Optional manual control of spot localization
- Detailed results comparison within single wells and spots
- Evaluation of the validity test through control spots
- Export of results in various formats
- LIS connectivity
Microblot-Array Reader
- Fast high-quality scanning and evaluation: 5 min. per full plate
- Scanning of selected wells
- Automated spot localization and image analysis
- Optimized for a 96-well microtiter plate format
Our MBA Categories
Autoimmune MBA kits
- Microblot-Array ANA includes a high number of antigens (44 antigens) that can be simultaneously detected in one sample. The kits are primarily intended for the identification of specific antibodies and confirmation of ELISA or other screening methods, allowing differentiation of systemic autoimmune diseases, such as myositis, scleroderma, systemic lupus, and others. The kits are optimized and validated for detection of specific IgG in human serum or plasma.
Used antigens:
- Group 1 (ANA): Jo-1, PL-7, PL-12, EJ, OJ, KS, YARS, ZoA, ZoB, SAE-1, SAE-2, SRP54, Mi-2, TIF1γ, MDA5, NXP2, PMScl 75, PMScl 100, CENP A, CENP B, Scl70, POLR3A, NOR90, PDGFR-β, Fibrillarin, Th/To, Ro52, Ro60, La, SmB, SmD, RNP A, RNP C, RNP 68/70, P0, Ku, Nucleolin, dsDNA, Histone, Nucleosome, PCNA; Supplementary antigens: M2, DFS70
- Group 2 (Myositis): Jo-1, PL-7, PL-12, EJ, OJ, KS, YARS, ZoA, ZoB, SAE-1, SAE-2, SRP54, Mi-2, TIF1γ, MDA5, NXP2; Supplementary antigens: Ro52, PMScl 75, PMScl 100, Ku
- Group 3 (Scleroderma): CENP A, CENP B, Scl70, POLR3A, NOR90, PDGFR-β, Fibrillarin, Th/To, PMScl 75, PMScl 100, RNP A, RNP C, RNP 68/70; Supplementary antigens: Ro52, Ku, M2
- Group 4 (SLE and other connective tissue diseases): dsDNA, Histone, Nucleosome, PCNA, SmB, SmD, RNP A, RNP C, RNP 68/70, P0, Ku, Nucleolin, Ro52, Ro60, La, NOR90
- Microblot-Array Autoimmune Gastroenteritis diseases: ASCA, DAG, tTG, IF, APCA, MPO
- Microblot-Array Liver profile: BPO, M2, Sp100, PML, gp210, LKM-1, LC-1, SLA/LP, RO52, ASGPR, Nup62, OGDC-E2, PDC-E2
Autoimmune Neurological disease:
- Paraneoplastic syndrome: Amyphysin, CV2, GAD65, Hu, MA1, MA2, MAG, Recoverin, Ri, SOX1, Titin, Tr, Yo, ZIC4, AChR, MusK, Aquaporin-4
- Limbic encephalitis: LGI1, CASPR2, NMDAR, AMPA1/2, GABA1/2
- Microblot-Array ANCA: PR3, MPO, GMB
Our Infectious Disease MBA kits:
- Gastrointestinal diseases: Helicobacter IgA, Helicobacter IgG, Yersinia IgA, Yersinia IgG
- Sexually transmitted diseases: Microblot–Array Chlamydia IgA, Microblot–Array Chlamydia IgG
- Respiratory infections: Bordetella IgA, Bordetella IgG, Bordetella IgM, Chlamydia IgA, Chlamydia IgG, Mycoplasma IgA, Mycoplasma IgG, Mycoplasma IgM
- Tick borne transmitted: Borrelia IgG, Borrelia IgM
- Herpetic infections: EBV, CMV, HSV 1+2, VZV, HHV–6, EBV IgA, EBV IgG, EBV IgM, CMV IgG, CMV IgM, HSV 1+2 IgG, HSV 1+2 IgM
- TORCH: CMV IgG, CMV IgM, HSV 1+2 IgG, HSV 1+2 IgM
- Tropical diseases: Dengue, Chikungunya, Zika, West Nile Fever, Plasmodium, Rickettsia, Leptospira
Microblot–Array (MBA) is a new generation of unique, immunoblot array in a microtiter plate format. It is designed for efficient multiplex diagnostics, enabling the simultaneous detection of multiple markers which can save time and lower costs. The technology eliminates the limited capacity of traditional BLOT strips and opens the opportunity for high throughput testing.
The main benefit of Microblot-Array kits is the high number of antigens which can be simultaneously detected in one sample. The kits are primarily intended for confirmation of ELISA or other screening methods. However, they also enable identification of specific antibodies and thus differentiation of systemic autoimmune diseases, such as myositis, scleroderma, systemic lupus, and others. The kits are optimized and validated for detection of specific IgG in human serum or plasma.
Main clinical areas covered:
- Infectious serology
- Autoimmunity
Microblot-Array
- Antigens spotted in triplicate – minimizing statistical variation
- Controls in each well
- 4 calibration spots to create a calibration curve
- Evaluation based on combination of positive antigen spots: qualitative, quantitative (U/ml) or semiquantitative (IP)
Microblot-Array Software
- Automated test identification
- Intuitive and user-friendly guiding throughout the results evaluation
- Complex image analysis
- Optional manual control of spot localization
- Detailed results comparison within single wells and spots
- Evaluation of the validity test through control spots
- Export of results in various formats
- LIS connectivity
Microblot-Array Reader
- Fast high-quality scanning and evaluation: 5 min. per full plate
- Scanning of selected wells
- Automated spot localization and image analysis
- Optimized for a 96-well microtiter plate format
Our MBA Categories
Autoimmune MBA kits
- Microblot-Array ANA includes a high number of antigens (44 antigens) that can be simultaneously detected in one sample. The kits are primarily intended for the identification of specific antibodies and confirmation of ELISA or other screening methods, allowing differentiation of systemic autoimmune diseases, such as myositis, scleroderma, systemic lupus, and others. The kits are optimized and validated for detection of specific IgG in human serum or plasma.
Used antigens:
- Group 1 (ANA): Jo-1, PL-7, PL-12, EJ, OJ, KS, YARS, ZoA, ZoB, SAE-1, SAE-2, SRP54, Mi-2, TIF1γ, MDA5, NXP2, PMScl 75, PMScl 100, CENP A, CENP B, Scl70, POLR3A, NOR90, PDGFR-β, Fibrillarin, Th/To, Ro52, Ro60, La, SmB, SmD, RNP A, RNP C, RNP 68/70, P0, Ku, Nucleolin, dsDNA, Histone, Nucleosome, PCNA; Supplementary antigens: M2, DFS70
- Group 2 (Myositis): Jo-1, PL-7, PL-12, EJ, OJ, KS, YARS, ZoA, ZoB, SAE-1, SAE-2, SRP54, Mi-2, TIF1γ, MDA5, NXP2; Supplementary antigens: Ro52, PMScl 75, PMScl 100, Ku
- Group 3 (Scleroderma): CENP A, CENP B, Scl70, POLR3A, NOR90, PDGFR-β, Fibrillarin, Th/To, PMScl 75, PMScl 100, RNP A, RNP C, RNP 68/70; Supplementary antigens: Ro52, Ku, M2
- Group 4 (SLE and other connective tissue diseases): dsDNA, Histone, Nucleosome, PCNA, SmB, SmD, RNP A, RNP C, RNP 68/70, P0, Ku, Nucleolin, Ro52, Ro60, La, NOR90
- Microblot-Array Autoimmune Gastroenteritis diseases: ASCA, DAG, tTG, IF, APCA, MPO
- Microblot-Array Liver profile: BPO, M2, Sp100, PML, gp210, LKM-1, LC-1, SLA/LP, RO52, ASGPR, Nup62, OGDC-E2, PDC-E2
Autoimmune Neurological disease:
- Paraneoplastic syndrome: Amyphysin, CV2, GAD65, Hu, MA1, MA2, MAG, Recoverin, Ri, SOX1, Titin, Tr, Yo, ZIC4, AChR, MusK, Aquaporin-4
- Limbic encephalitis: LGI1, CASPR2, NMDAR, AMPA1/2, GABA1/2
- Microblot-Array ANCA: PR3, MPO, GMB
Our Infectious Disease MBA kits:
- Gastrointestinal diseases: Helicobacter IgA, Helicobacter IgG, Yersinia IgA, Yersinia IgG
- Sexually transmitted diseases: Microblot–Array Chlamydia IgA, Microblot–Array Chlamydia IgG
- Respiratory infections: Bordetella IgA, Bordetella IgG, Bordetella IgM, Chlamydia IgA, Chlamydia IgG, Mycoplasma IgA, Mycoplasma IgG, Mycoplasma IgM
- Tick borne transmitted: Borrelia IgG, Borrelia IgM
- Herpetic infections: EBV, CMV, HSV 1+2, VZV, HHV–6, EBV IgA, EBV IgG, EBV IgM, CMV IgG, CMV IgM, HSV 1+2 IgG, HSV 1+2 IgM
- TORCH: CMV IgG, CMV IgM, HSV 1+2 IgG, HSV 1+2 IgM
- Tropical diseases: Dengue, Chikungunya, Zika, West Nile Fever, Plasmodium, Rickettsia, Leptospira
